Simultaneous Pharmacokinetic Evaluation of Pantoprazole and Vitamin B Complex for Assessing Drug–Drug Interactions in Healthy Bangladeshi Adults by a Newly Developed and Validated HPLC Method

The present study has been designed to evaluate the impact of co-administration pantoprazole (PNT) with vitamin B (VTB) complex (VTB comprising VTB1, VTB6, and VTB12 in this study) on pharmacokinetic behavior. In study, HPLC-based sensitive efficient methods for simultaneous determination human plasma were developed per US-FDA bioanalytical standards. parameters PNT, also evaluated when medicines administered alone co-administered. Following linearity, it was observed that retention times 6.8 ± 0.2, 2.7 0.1, 5.5 3.8 0.1 min, respectively, over range 1−100 μg/mL. For all analytes at lower limit quantification other values, intra-assay inter-assay bias within 15% 13.5%, respectively. They barely interacted PNT VTB samples physical combinations through vitro tests. Moreover, pharmacokinetics treatment did not significantly alter characteristics PNT. Therefore, current work’s results might help assess drug–drug interactions may be applied bioequivalence studies therapeutic drug monitoring.